منابع مشابه
The unresolved safety concerns of bovine thrombin
A recent review has suggested that bovine thrombin is not associated with an increased risk of bleeding in surgical populations. In spite of extremely limited evidence available, many valuable resources (e.g. safety surveillance and post-marketing programs, case reports) were excluded in reaching this conclusion. While waiting for the adequately powered, controlled clinical trials to address th...
متن کاملThe purification and properties of bovine thrombin.
A method for the purification of bovine thrombin (EC 3.4.4.13) from Parke-Davis topical thrombin is described. The purified preparation has a constant specific activity although it has previously been shown to contain at least two enzymatically active proteins of similar molecular weights and amino acid composition. The average molecular weight of this purified material as determined by gel fil...
متن کاملBovine Thrombin and Activated Factor X
Parke-Davis bovine thrombin was resolved into three distinct protein peaks on a Sephadex G-200 column. A stepwise chromatographic technique on diethylaminoethyl (DEAE) cellulose was developed for the complete separation and partial purification of thrombin and activated Factor X. Thrombin was quantitatively eluted in the first protein peak with 0.1 M NaCl, pH 7.0. A second peak with residual th...
متن کاملSome physicochemical properties of bovine thrombin.
The physical properties of bovine prothrombin preparations have been described in this journal (1). Although methods for obtaining bovine thrombin are now also available (a), the physical properties, including the molecular weight, have not been described. Here we report values for the sedimentation coefficient, diffusion coefficient, intrinsic viscosity, frictional ratio, molecular weight, and...
متن کاملBovine thrombin. Purification and certain properties.
An examination is made of the fractionation of a crude thrombin preparation obtained by bioactivation, namely, Parke Davis thrombin, topical. Columns of carboxylic resin, cellulose phosphate, dlethylaminoethyl cellulose, and Sephadex G-100 were studied. The starting material and all products obtained by using only carboxylic resin or cellulose phosphate are shown to be impure and to contain a n...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99419-6